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5 Horizontal Gene Transfer

Horizontal Gene Transfer in Bacteria: A Dynamic Force in Evolution

Learning Objectives

Upon completing this chapter, you will be able to:

  • Define Horizontal Gene Transfer (HGT) and differentiate it from vertical gene transfer.
  • Explain the general significance of HGT in bacterial evolution, adaptation (e.g., antibiotic resistance), and genetic diversity.
  • Describe the process of transformation, including the concept of competence and how naked DNA is taken up and integrated into a recipient bacterium’s genome.
  • Describe the process of transduction, explaining the role of bacteriophages and distinguishing between generalized and specialized transduction.
  • Describe the process of conjugation, including the roles of the F-plasmid (or conjugative plasmids), sex pili, and the mechanism of DNA transfer, including Hfr transfer.
  • Identify the key molecular events and components involved in each of the three HGT mechanisms.
  • Discuss the broader implications of HGT in areas such as medicine and biotechnology.

Introduction to Horizontal Gene Transfer (HGT)

Bacteria, despite their seemingly simple cellular structure, possess remarkable adaptability. A key driver of this adaptability is Horizontal Gene Transfer (HGT), also known as lateral gene transfer. Unlike vertical gene transfer, where genetic material is passed down from parent to offspring during reproduction, HGT allows bacteria to acquire new genetic material from other bacteria – even those that are distantly related – within the same generation. This process plays a crucial role in bacterial evolution, enabling rapid acquisition of new traits such as antibiotic resistance, virulence, and the ability to metabolize novel substrates. HGT is a significant force shaping microbial communities and has profound implications for medicine, agriculture, and biotechnology.

The genetic material transferred during HGT can range from small DNA fragments to large segments containing multiple genes. Once incorporated into the recipient bacterium’s genome, these newly acquired genes can be expressed and passed on to subsequent generations through vertical gene transfer. This constant shuffling and sharing of genetic information contribute to the incredible genetic diversity observed in the bacterial world.

There are three primary mechanisms by which HGT occurs in bacteria: transformation, transduction, and conjugation. Each mechanism involves a distinct process for the introduction of foreign DNA into a recipient bacterial cell.

a) Transformation is when DNA enters into a cell and is incorporated into the genome. B) transduction is when a virus injects DNA into a cell and this DNA is incorporated into the genome. C) Conjugation is when one bacterial cell copies its plasmid and sends that copy to another bacterial cell via a pilus (bridge of cytoplasm).
Figure x.x: There are three prokaryote-specific mechanisms leading to horizontal gene transfer in prokaryotes. a) In transformation, the cell takes up DNA directly from the environment. The DNA may remain separate as a plasmid or be incorporated into the host genome. b) In transduction, a bacteriophage injects DNA that is a hybrid of viral DNA and DNA from a previously infected bacterial cell. c) In conjugation, DNA is transferred between cells through a cytoplasmic bridge after a conjugation pilus draws the two cells close enough to form the bridge.

Transformation: Picking Up Naked DNA

Transformation is the process by which a bacterium takes up free or “naked” DNA from its surrounding environment. This DNA is typically released from other bacterial cells that have lysed (burst open), liberating their cellular contents, including their genetic material.

The Basics of Transformation:

  1. Competence: Not all bacteria can readily take up exogenous DNA. Bacteria capable of transformation are said to be competent or in a state of competence. Competence can be a transient, genetically programmed state influenced by environmental factors such as nutrient availability, cell density (quorum sensing), and stress conditions. Some bacteria are naturally competent, while others can be induced to become competent in the laboratory through chemical treatments (e.g., calcium chloride) or electroporation.
  2. DNA Binding and Uptake: Competent cells possess specific DNA receptor proteins on their cell surface that bind to the free DNA fragments. Following binding, the DNA is transported across the cell membrane and cell wall into the cytoplasm. In some bacteria, double-stranded DNA is converted into a single-stranded molecule during uptake, while in others, double-stranded DNA enters the cell.
  3. Integration into the Genome: Once inside the recipient cell, the foreign DNA must be stably maintained and replicated to be passed on. This usually occurs through homologous recombination, where the newly acquired DNA segment replaces a similar, pre-existing segment in the recipient’s chromosome. This requires sequence similarity between the incoming DNA and the host genome. If the DNA is a plasmid (a small, circular DNA molecule capable of self-replication), it may persist and replicate independently of the chromosome.

Transformation is a significant mechanism for acquiring genes that can confer advantages such as antibiotic resistance or the ability to utilize new food sources. It was famously demonstrated by Frederick Griffith in 1928, providing early evidence that DNA is the carrier of genetic information.

Beyond its natural occurrence, transformation is a cornerstone technique in molecular biology laboratories, deliberately used to introduce specific genes into bacteria. Scientists can artificially induce competence in bacteria like E. coli, making them permeable to foreign DNA. A common application, often performed in educational settings, is the transformation of E. coli cells with a plasmid such as pGLO. This plasmid carries genes for traits like ampicillin resistance and the gene for Green Fluorescent Protein (GFP). Successful transformation allows the bacteria to express these new genes, resulting in observable characteristics like antibiotic resistance and, in the case of pGLO, fluorescence under UV light, demonstrating the power of this HGT mechanism for genetic engineering.

Transduction: Virus-Mediated Gene Transfer

Transduction involves the transfer of bacterial DNA from one bacterium to another via a bacteriophage (often shortened to “phage”), which is a virus that infects bacteria. Bacteriophages hijack the host cell’s machinery to replicate themselves. During this process, bacterial DNA can sometimes be mistakenly packaged into new phage particles.

The Basics of Transduction:

  1. Phage Infection and Replication: A bacteriophage attaches to a donor bacterial cell and injects its own genetic material. The phage then typically directs the host cell to produce new phage components (proteins and nucleic acids).
  2. Mispackaging of Bacterial DNA: During the assembly of new phage particles, fragments of the host bacterium’s chromosome or plasmids can occasionally be accidentally packaged into a phage head instead of, or along with, the phage’s own genetic material. This results in a transducing phage particle.
  • Generalized Transduction: Any segment of the bacterial DNA has a roughly equal chance of being packaged into a phage. This occurs when the phage enters the lytic cycle, leading to the degradation of the host chromosome into fragments, some of which are then mistakenly packaged.
  • Specialized Transduction: Only specific genes located near the phage integration site on the bacterial chromosome are transferred. This occurs with temperate phages that can integrate their DNA into the host chromosome (prophage) and enter a lysogenic cycle. When the prophage is induced to enter the lytic cycle and excise itself from the host chromosome, it may occasionally carry adjacent bacterial genes with it.
  1. Infection of a Recipient Cell: The transducing phage particle, carrying bacterial DNA, then infects a new recipient bacterial cell.
  2. DNA Integration: The transferred bacterial DNA is injected into the recipient cell. As with transformation, this DNA can then be incorporated into the recipient’s genome via homologous recombination, conferring new traits to the recipient cell.

Transduction is a powerful mechanism for gene transfer and can occur between different bacterial species if they are susceptible to infection by the same phage. It plays a significant role in the spread of virulence factors and antibiotic resistance genes.

Conjugation: Bacterial “Mating”

Conjugation is a process that involves the direct transfer of DNA from one bacterial cell to another through cell-to-cell contact. It is often likened to bacterial “mating” and typically requires a specialized transferable DNA element, most commonly a conjugative plasmid (such as the F-plasmid or fertility factor in E. coli).

Diagram of conjugation. 1: Pilus of donor cell attaches to recipient cell. The donor cell contains a plasmid labeled F plasmid; the cell is labeled F+ donor cell. The recipient cell is labeled F- recipient cell and does not contain a plasmid. A bridge between them is labeled pilus. 2: Pilus contracts, drawing cells together to make contact with one another. 3: One strand of F plasmid DNA transfers from donor cell to recipient cell. 4: Donor synthesizes complementary strand to restore plasmid. Recipient synthesizes complementary strand to become F+ cell pith pilus. Both cells are now labeled F+ and contain a small circular plasmid.
Figure x.x: Typical conjugation of the F plasmid from an F+ cell to an F− cell is brought about by the conjugation pilus bringing the two cells into contact. A single strand of the F plasmid is transferred to the F− cell, which is then made double stranded.

The Basics of Conjugation:

  1. Pilus Formation: The donor cell, which carries the conjugative plasmid, produces a structure called a sex pilus (or F-pilus). This pilus is a protein appendage that extends from the donor cell and makes contact with a recipient cell that lacks the plasmid.
  2. Cell-to-Cell Contact and Mating Bridge: The pilus retracts, drawing the two cells close together. A stable mating bridge or conjugation tube then forms between the donor and recipient cells, providing a channel for DNA transfer.
  3. DNA Transfer: The conjugative plasmid in the donor cell initiates a process of rolling circle replication. One strand of the plasmid DNA is nicked, and this single strand is then transferred through the mating bridge into the recipient cell. The donor cell simultaneously synthesizes a complementary strand to replace the transferred strand, thus retaining a copy of the plasmid.
  4. Synthesis of a Complementary Strand in the Recipient: Once the single strand of plasmid DNA enters the recipient cell, it serves as a template for the synthesis of a complementary strand, resulting in a complete, double-stranded plasmid. The recipient cell is now also a donor cell (e.g., F+ in the case of the F-plasmid) and can transfer the plasmid to other recipient cells.
  5. Hfr (High-Frequency Recombination) Transfer: In some cases, the conjugative plasmid can integrate into the bacterial chromosome. Cells with an integrated plasmid are called Hfr cells. During conjugation involving an Hfr cell, a portion of the bacterial chromosome, along with part of the plasmid, can be transferred to the recipient. The amount of chromosomal DNA transferred depends on how long the cells remain in contact. The transferred chromosomal DNA can then be integrated into the recipient’s chromosome via homologous recombination.

Conjugation is a highly efficient mechanism for transferring genetic material, including large plasmids carrying multiple genes (e.g., for antibiotic resistance, metabolic pathways). It can occur between different bacterial species and even across broader taxonomic groups, contributing significantly to the rapid dissemination of important traits within microbial populations.

Significance of HGT

Horizontal gene transfer is a cornerstone of bacterial evolution and adaptation. It allows bacteria to rapidly acquire new functions, respond to environmental pressures, and exploit new ecological niches. The spread of antibiotic resistance genes through HGT poses a major global health challenge. Understanding the mechanisms of HGT is therefore critical for developing strategies to combat infectious diseases, for utilizing bacteria in bioremediation, and for various biotechnological applications. It highlights the dynamic and interconnected nature of the microbial world.

Chapter Summary

Horizontal Gene Transfer (HGT) is a crucial mechanism for bacterial evolution, allowing bacteria to acquire new genetic material from other bacteria within the same generation, distinct from the parent-to-offspring vertical gene transfer. This process significantly contributes to bacterial adaptability, including the spread of traits like antibiotic resistance and virulence. HGT occurs primarily through three distinct mechanisms:

  1. Transformation: Bacteria take up free or “naked” DNA released from other lysed cells in their environment. This process requires the recipient cell to be in a state of competence, allowing DNA binding, uptake, and subsequent integration into its genome, often via homologous recombination.

  2. Transduction: Genetic material is transferred from a donor bacterium to a recipient bacterium via a bacteriophage (a virus that infects bacteria). During phage replication, bacterial DNA can be mistakenly packaged into new phage particles. Generalized transduction allows any bacterial gene to be transferred, while specialized transduction involves the transfer of specific genes located near the phage integration site in the bacterial chromosome.

  3. Conjugation: DNA is transferred directly from a donor cell to a recipient cell through cell-to-cell contact, often referred to as bacterial “mating.” This process typically involves a conjugative plasmid (like the F-plasmid), which codes for the formation of a sex pilus that initiates contact and a mating bridge for DNA transfer via rolling circle replication. High-frequency recombination (Hfr) cells, with the plasmid integrated into their chromosome, can also transfer chromosomal DNA.

Overall, HGT is a powerful evolutionary force that drives genetic diversity and rapid adaptation in bacterial populations. Understanding these mechanisms is vital for addressing challenges like antibiotic resistance and for leveraging bacterial genetics in biotechnology.

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Microbial Genetics (Dr.B) Copyright © 2025 by Graham Boorse is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License, except where otherwise noted.